Publications

Milk Glycoproteome

• Nwosu CC, Seipert R, Strum JS, Hua SS, An HJ, Zivkovic AM, German JB, Lebrilla CB. Simultaneous and Extensive Site-specific N- and O-Glycosylation Analysis in Protein Mixtures. J Proteome Res. 2011 Apr 6. [Epub ahead of print]
• An HJ, Lebrilla CB. Structure elucidation of native N- and O-linked glycans by tandem mass spectrometry (tutorial). Mass Spectrom Rev. 2010 Nov 22. [Epub ahead of print]
• Nwosu CC, Strum JS, An HJ, Lebrilla CB. Enhanced detection and identification of glycopeptides in negative ion mode mass spectrometry. Anal Chem. 2010 Dec 1;82(23):9654-62. 
• Froehlich JW, Chu CS, Tang N, Waddell K, Grimm R, Lebrilla CB. Label-free liquid chromatography-tandem mass spectrometry analysis with automated phosphopeptide enrichment reveals dynamic human milk protein phosphorylation during lactation. Anal Biochem. 2011 Jan 1;408(1):136-46.
• Froehlich JW, Dodds ED, Barboza M, et al. Glycoprotein Expression in Human Milk during Lactation. J Agric Food Chem 2010; 58(10): 6440-8. 
• Dodds, E. D., R. R. Seipert, et al. (2009). "Analytical performance of immobilized pronase for glycopeptide footprinting and implications for surpassing reductionist glycoproteomics." J Proteome Res 8(2): 502-12.
• An HJ, Froehlich JW, Lebrilla CB. Determination of glycosylation sites and site-specific heterogeneity in glycoproteins. Curr Opin Chem Biol. 2009 Oct;13(4):421-6.
• Tao N, DePeters EJ, Freeman S, German JB, Grimm R, Lebrilla CB. Bovine milk glycome. J Dairy Sci 2008;91:3768-78. 
• Seipert, R. R., E. D. Dodds, et al. (2008). "Factors that influence fragmentation behavior of N-linked glycopeptide ions." Anal Chem 80(10): 3684-92.
• Dodds, E. D., J. B. German, et al. (2007). "Enabling MALDI-FTICR-MS/MS for high-performance proteomics through combination of infrared and collisional activation." Anal Chem 79(24): 9547-56.

Milk Oligosaccharides

• Wu S, Grimm R, German JB, Lebrilla CB. Annotation and structural analysis of sialylated human milk oligosaccharides. J Proteome Res. 2011 Feb 4;10(2):856-68.
• Tao N, Wu S, Kim J, An HJ, Hinde K, Power ML, Gagneux P, German JB, Lebrilla CB. Evolutionary Glycomics: Characterization of Milk Oligosaccharides in Primates. J Proteome Res. 2011 Feb 22. [Epub ahead of print]
• Sela DA, Li Y, Lerno L, Wu S, Marcobal AM, German JB, Chen X, Lebrilla CB, Mills DA. An infant-associated bacterial commensal utilizes breast milk sialyloligosaccharides. J Biol Chem. 2011 Feb 2. [Epub ahead of print]
• Barile D, Marotta M, Chu C, Mehra R, Grimm R, Lebrilla CB, German JB. Neutral and acidic oligosaccharides in Holstein-Friesian colostrum during the first 3 days of lactation measured by high performance liquid chromatography on a microfluidic chip and time-of-flight mass spectrometry. J. Dairy Sci. 2010; 93: 3940-3949.
• Zivkovic AM, German JB, Lebrilla CB, Mills DA. Microbes and Health Sackler Colloquium: Human milk glycobiome and its impact on the infant gastrointestinal microbiota. Proc Natl Acad Sci U S A. 2010 Aug 2. [Epub ahead of print]
• Sela DA, Mills DA. Nursing our microbiota: molecular linkages between bifidobacteria and milk oligosaccharides. Trends Microbiol. 2010 Jul;18(7):298-307.
• Wu S, Tao N, German JB, Grimm R, Lebrilla CB. The development of an annotated library of neutral human milk oligosaccharides. J Proteome Res. 2010 Aug 6;9(8):4138-51.
• Tao N, Ochonicky KL, German JB, Donovan SM, Lebrilla CB. Structural determination and daily variations of porcine milk oligosaccharides. J Agric Food Chem 2010;58:4653-9. 
• Marcobal A, Barboza M, Froehlich JW, Block, D., German, J.B., Lebrilla, C.B., Mills, D.A. Consumption of Human Milk Oligosaccharides by Gut-Related Microbes. J Agric Food Chem 2010; 58(9): 5334-40.
• Niñonuevo MR, Lebrilla CB. Mass spectrometric methods for analysis of oligosaccharides in human milk. Nutr Rev. 2009 Nov;67 Suppl 2:S216-26.
• Tao N, DePeters EJ, German JB, Grimm R, Lebrilla CB. Variations in bovine milk oligosaccharides during early and middle lactation stages analyzed by high-performance liquid chromatography-chip/mass spectrometry. J Dairy Sci 2009; 92:2991-3001. 
• Barboza M, Sela DA, Pirim C, et al. Glycoprofiling bifidobacterial consumption of galacto-oligosaccharides by mass spectrometry reveals strain-specific, preferential consumption of glycans. Appl Environ Microbiol 2009;75:7319-25. 
• Barile D, Tao N, Lebrilla CB, Coisson JD, Arlorio M, German JB. Permeate from cheese whey ultrafiltration is a source of milk oligosaccharides. Int Dairy J 2009;19:524-530.
• Sela, D. A., J. Chapman, et al. (2008). "The genome sequence of Bifidobacterium longum subsp. infantis reveals adaptations for milk utilization within the infant microbiome." Proc Natl Acad Sci U S A 105(48): 18964-9.
• German, J. B., S. L. Freeman, et al. (2008). "Human milk oligosaccharides: evolution, structures and bioselectivity as substrates for intestinal bacteria."Nestle Nutr Workshop Ser Pediatr Program 62: 205-18; discussion 218-22.
• Ninonuevo, M. R., P. D. Perkins, et al. (2008). "Daily variations in oligosaccharides of human milk determined by microfluidic chips and mass spectrometry." J Agric Food Chem 56(2): 618-26.
• Seipert, R. R., M. Barboza, et al. (2008). "Analysis and quantitation of fructooligo-saccharides using matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry." Anal Chem 80(1): 159-65.
• Ward, R. E., M. Ninonuevo, et al. (2007). "In vitro fermentability of human milk oligosaccharides by several strains of bifidobacteria." Mol Nutr Food Res 51(11): 1398-405.
• LoCascio, R. G., M. R. Ninonuevo, et al. (2007). "Glycoprofiling of bifidobacterial consumption of human milk oligosaccharides demonstrates strain specific, preferential consumption of small chain glycans secreted in early human lactation." J Agric Food Chem 55(22): 8914-9.
• Ninonuevo, M. R., R. E. Ward, et al. (2007). "Methods for the quantitation of human milk oligosaccharides in bacterial fermentation by mass spectrometry." Anal Biochem 361(1): 15-23.
• Ninonuevo, M. R., Y. Park, et al. (2006). "A strategy for annotating the human milk glycome." J Agric Food Chem 54(20): 7471-80.
• Ward, R. E., M. Ninonuevo, et al. (2006). "In vitro fermentation of breast milk oligosaccharides by Bifidobacterium infantis and Lactobacillus gasseri." Appl Environ Microbiol 72(6): 4497-9.

Bioactive Glycopeptides

• Froehlich JW, Dodds ED, Barboza M, et al. Glycoprotein Expression in Human Milk during Lactation. J Agric Food Chem 2010; 58(10): 6440-8. 
• An HJ, Froehlich JW, Lebrilla CB. Determination of glycosylation sites and site-specific heterogeneity in glycoproteins. Curr Opin Chem Biol. 2009 Oct;13(4):421-6.
• Dodds, E. D., R. R. Seipert, et al. (2009). "Analytical performance of immobilized pronase for glycopeptide footprinting and implications for surpassing reductionist glycoproteomics." J Proteome Res 8(2): 502-12.
• Seipert, R. R., E. D. Dodds, et al. (2008). "Factors that influence fragmentation behavior of N-linked glycopeptide ions." Anal Chem 80(10): 3684-92.
• Dodds, E. D., J. B. German, et al. (2007). "Enabling MALDI-FTICR-MS/MS for high-performance proteomics through combination of infrared and collisional activation." Anal Chem 79(24): 9547-56.

Glycolipids

• Lerno LA, German JB, Lebrilla CB. Method for the Identification of Lipid Classes Based on Referenced Kendrick Mass Analysis. Anal Chem 2010; 82(10): 4236-45.
• Schombs M, Park FE, Du W, Kulkarni SS, Gervay-Hague J. One-Pot Syntheses of Immunostimulatory Glycolipids.  J Org Chem. 2010 Apr 13. 

Synthesis of Novel Glycans

• Lau K, Thon V, Yu H, Ding L, Chen Y, Muthana MM, Wong D, Huang R, Chen X. Highly efficient chemoenzymatic synthesis of beta1-4-linked galactosides with promiscuous bacterial beta1-4-galactosyltransferases. Chem Commun (Camb). 2010 Jul 12.
• Schombs M, Park FE, Du W, Kulkarni SS, Gervay-Hague J. One-Pot Syntheses of Immunostimulatory Glycolipids.  J Org Chem. 2010 Apr 13. 
• Horn EJ, Gervay-Hague J. Efficient method for the preparation of peracetylated Neu5Ac2en by flash vacuum pyrolysis. J Org Chem. 2009 Jun 5;74(11):4357-9.
• Saludes JP, Ames JB, Gervay-Hague J. Synthesis and structural characterization of sialic acid-glutamic acid hybrid foldamers as conformational surrogates of alpha-2,8-linked polysialic acid. J Am Chem Soc. 2009 Apr 22;131(15):5495-505.
• Cheng J, Yu H, Lau K, Huang S, Chokhawala HA, Li Y, Tiwari VK, Chen X: Multifunctionality of Campylobacter jejuni sialyltransferase CstII: characterization of GD3/GT3 oligosaccharide synthase, GD3 oligosaccharide sialidase, and trans-sialidase activities. Glycobiology 2008, 18:686-697.
• Sun M, Li Y, Chokhawala HA, Henning R, Chen X: N-Terminal 112 amino acid residues are not required for the sialyltransferase activity of Photobacterium damsela alpha2,6-sialyltransferase. Biotechnol Lett 2008, 30:671-676.
• Chokhawala HA, Huang S, Lau K, Yu H, Cheng J, Thon V, Hurtado-Ziola N, Guerrero JA, Varki A, Chen X: Combinatorial chemoenzymatic synthesis and high-throughput screening of sialosides. ACS Chem Biol 2008, 3:567-576.
• Kulkarni SS, Gervay-Hague J. Two-step synthesis of the immunogenic bacterial glycolipid BbGL1. Org Lett. 2008 Nov 6;10(21):4739-42.
• Li Y, Sun M, Huang S, Yu H, Chokhawala HA, Thon V, Chen X: The Hd0053 gene of Haemophilus ducreyi encodes an alpha2,3-sialyltransferase. Biochem Biophys Res Commun 2007, 361:555-560.
• Du W, Kulkarni SS, Gervay-Hague J. Efficient, one-pot syntheses of biologically active alpha-linked glycolipids. Chem Commun (Camb). 2007 Jun 21;(23):2336-8.
• Yu H, Chokhawala HA, Varki A, Chen X: Efficient chemoenzymatic synthesis of biotinylated human serum albumin-sialoglycoside conjugates containing O-acetylated sialic acids. Org Biomol Chem 2007, 5:2458-2463.
• Yu H, Chokhawala HA, Huang S, Chen X: One-pot three-enzyme chemoenzymatic approach to the synthesis of sialosides containing natural and non-natural functionalities. Nat Protoc 2006, 1:2485-2492.
• Yu H, Chen X: Aldolase catalyzed synthesis of beta-D-Gal p -(1-9)-D-KDN: A novel acceptor for sialyltransferases. Org Lett 2006, 8:2393-2396.
• Yu H, Huang S, Chokhawala H, Sun M, Zheng H, Chen X: Highly efficient chemoenzymatic synthesis of naturally occurring and non-natural alpha-2,6-linked sialosides: A P. damsela alpha-2,6-sialyltransferase with extremely flexible donor-substrate specificity. Angew Chem Int Ed Engl 2006, 45:3938-3944.

Infant Microbiome

• Wu S, Grimm R, German JB, Lebrilla CB. Annotation and structural analysis of sialylated human milk oligosaccharides. J Proteome Res. 2011 Feb 4;10(2):856-68.
• Sela DA, Li Y, Lerno L, Wu S, Marcobal AM, German JB, Chen X, Lebrilla CB, Mills DA. An infant-associated bacterial commensal utilizes breast milk sialyloligosaccharides. J Biol Chem. 2011 Feb 2. [Epub ahead of print]
• Zivkovic AM, German JB, Lebrilla CB, Mills DA. Microbes and Health Sackler Colloquium: Human milk glycobiome and its impact on the infant gastrointestinal microbiota. Proc Natl Acad Sci U S A. 2010 Aug 2. 
• Sela DA, Mills DA. Nursing our microbiota: molecular linkages between bifidobacteria and milk oligosaccharides. Trends Microbiol. 2010 Jul;18(7):298-307.
• Wu S, Tao N, German JB, Grimm R, Lebrilla CB. The development of an annotated library of neutral human milk oligosaccharides. J Proteome Res. 2010 Jun 28. [Epub ahead of print]
• Marcobal A, Barboza M, Froehlich JW, Block, D., German, J.B., Lebrilla, C.B., Mills, D.A. Consumption of Human Milk Oligosaccharides by Gut-Related Microbes. J Agric Food Chem 2010; 58(9): 5334-40.
• Barboza M, Sela DA, Pirim C, et al. Glycoprofiling bifidobacterial consumption of galacto-oligosaccharides by mass spectrometry reveals strain-specific, preferential consumption of glycans. Appl Environ Microbiol 2009;75:7319-25. 
• Sela, D. A., J. Chapman, et al. (2008). "The genome sequence of Bifidobacterium longum subsp. infantis reveals adaptations for milk utilization within the infant microbiome." Proc Natl Acad Sci U S A 105(48): 18964-9.
• German, J. B., S. L. Freeman, et al. (2008). "Human milk oligosaccharides: evolution, structures and bioselectivity as substrates for intestinal bacteria."Nestle Nutr Workshop Ser Pediatr Program 62: 205-18; discussion 218-22.
• Ward, R. E., M. Ninonuevo, et al. (2007). "In vitro fermentability of human milk oligosaccharides by several strains of bifidobacteria." Mol Nutr Food Res 51(11): 1398-405.
• LoCascio, R. G., M. R. Ninonuevo, et al. (2007). "Glycoprofiling of bifidobacterial consumption of human milk oligosaccharides demonstrates strain specific, preferential consumption of small chain glycans secreted in early human lactation." J Agric Food Chem 55(22): 8914-9.
• Ninonuevo, M. R., R. E. Ward, et al. (2007). "Methods for the quantitation of human milk oligosaccharides in bacterial fermentation by mass spectrometry."Anal Biochem 361(1): 15-23.
• Ward, R. E., M. Ninonuevo, et al. (2006). "In vitro fermentation of breast milk oligosaccharides by Bifidobacterium infantis and Lactobacillus gasseri." Appl Environ Microbiol 72(6): 4497-9.

Host-Microbe Interactions

• Cooke CL, An HJ, Kim J, Canfield DR, Torres J, Lebrilla CB, Solnick JV. Modification of gastric mucin oligosaccharide expression in rhesus macaques after infection with Helicobacter pylori. Gastroenterology. 2009 Sep;137(3):1061-71, 1071.e1-8.
• Sun YS, Landry JP, Fei YY, Zhu X, Luo JT, Wang XB, Lam KS: Macromolecular scaffolds for immobilizing small molecule microarrays in label-free detection of protein-ligand interactions on solid support. Anal Chem 2009, Jul 1;81(13):5373-80.
• Landry JP, Sun YS, Guo XW, Zhu XD: Protein reactions with surface-bound molecular targets detected by oblique-incidence reflectivity difference microscopes. Appl Opt 2008, 47:3275-3288. 
• Sun YS, Landry JP, Fei YY, Zhu XD, Luo JT, Wang XB, Lam KS: Effect of fluorescently labeling protein probes on kinetics of protein-ligand reactions. Langmuir 2008, 24:13399-13405.
• Fei YY, Landry JP, Sun YS, Zhu XD, Luo JT, Wang XB, Lam KS: A novel high-throughput scanning microscope for label-free detection of protein and small-molecule chemical microarrays. Rev Sci Instrum 2008, 79:013708.
• Desai PT, Walsh MK, Weimer BC: Solid-phase capture of pathogenic bacteria by using gangliosides and detection with real-time PCR. Appl Environ Microbiol 2008, 74:2254-2258.
• Zhu X, Landry JP, Sun YS, Gregg JP, Lam KS, Guo X: Oblique-incidence reflectivity difference microscope for label-free high-throughput detection of biochemical reactions in a microarray format. Appl Opt 2007, 46:1890-1895.
• Landry JP, Zhu XD, Gregg JP: Label-free detection of microarrays of biomolecules by oblique-incidence reflectivity difference microscopy. Opt Lett 2004, 29:581-583.